Cover different classification schemes for grouping bacteria, especially the use of the gram stain 2. Gram staining involves a fourpart process, which includes. Differential stain distinguishes between different groups of bacteria based on their staining ability. The acid fast stain was discovered by ehrlich, who found that after staining with. Flood slide with crystal or gentian violet 60 seconds. In addition this stain also allows determination of cell morphology, size, and arrangement.
Learn for free about math, art, computer programming, economics, physics, chemistry, biology, medicine, finance, history, and more. Find powerpoint presentations and slides using the power of, find free presentations research about gram staining ppt. Gram staining gram staining is most widely used differential staining in microbiology. View and download powerpoint presentations on staining techniques ppt. We have uploaded a genuine pdf ebook copy of this book to our online file repository so that. Khan academy is a nonprofit with the mission of providing a free, worldclass education for anyone, anywhere. A rapid method for distinction between gram negative and grampositive bacteria by means of a 3% solution of potassium hydroxide is tested on 71 gram positive and 55 gram negative bacterial strains.
The gram stain reaction, named after the danish bacteriologist hans christian gram, was originally devised in 1882. The best way for students to print out the powerpoint show is to download the pdf version. Ppt gram staining powerpoint presentation, free download. Crystal violet primary stain for 1 minrinse gram s iodine mordant for 1 minrinse acetonealcohol decolorizer for 5 seconds safranin counterstain for 1 minrinse gram negative red and gram positive blue assess gram reaction a. A staining technique used to classify bacteria in which a bacterial specimen is first stained with crystal violet, then treated with an. Gather all equipment and supplies gram stain microscope s heat source sterile swab culture or specimen microscope gram stain procedure 2. Prescotts microbiology 9th edition pdf free download.
Alright, now in this part of the article, you will be able to access the free pdf download of prescotts microbiology 9th edition pdf using our direct links mentioned at the end of this article. We chose the most two resistant isolates to zinc for further investigations. Who developed gram stains hans christian gram danish physician 1884 original purpose was to discern the difference between two types of pnuemonia. It is used to differentiate between gram positive organisms and gram negative organisms. An ebook reader can be a software application for use on a computer such as microsofts free reader application, or a booksized computer this is used solely as a reading device such as nuvomedias rocket ebook. Ppt gram stain powerpoint presentation free to view.
Introduction as bacteria consist of clear protoplasmic matter, differing but slightly in refractive index from the medium in which they are growing, it is difficult with the ordinary microscope, except when special methods of illumination are used, to set them in the unstained condition. The staining technique, developed in the late 1700s by christian gram classifies the rigid cell walled bacteria into one of two groups based on whether they are able to resist the decolorizing action of an alcoholic. Principle, procedure, interpretation, examples and animation last updated. Microbiology mcq question paper pdf in this article i have provided multiple choice questions mcqs of microbiology in pdf which is very important for those who are preparing for competitive exams like neet, gpat, aiims. Basic microbiology lab reporting gram staining staining. The name comes from the danish bacteriologist hans christian gram, who developed the technique. Gram staining for differentiating bacterial species youtube. Affordable and search from millions of royalty free images, photos and vectors. Gram staining is a differential staining technique that provides an easy differentiation of bacteria into one of two groups. Gram staining is an essential procedure used in the identification of bacteria and is frequently the only method required for studying their morphology. In indirect, or negative, staining, smears are produced by mixing material with india ink or acidic dyes such as nigrosine. Selection of suitable culture media based on gram stain finding.
To use and relate the gram stain to the study of bacterial cell morphology, and as an important step in the identification of a bacterial species. Christian gram in 1884, and categorizes bacteria according to their gram character gram positive or gram negative. Practical gram staining practical gram staining assist prof dr. Theyll give your presentations a professional, memorable appearance the kind of sophisticated look that todays audiences expect. Gram staining is still the cornerstone of bacterial identification and taxonomic division. The gram reaction reflects fundamental differences in the biochemical and structural properties of bacteria. Find powerpoint presentations and slides using the power of, find free presentations research about staining techniques ppt. Free microbiology photos of gram stained slides viewed through compound microscope and with naked eye. Beyer individual supplies microscope slide clothes pin inoculating loop or wood splint paper towels shared supplies crystal violet stain safranin red stain grams iodine ethyl alcohol denatured water bottle rinsing basin alcohol burner wax pencil matches microscope create a staining area on your microscope slide obtain a clean microscope slide draw a. Microbiology lecture 2 gram positive and gram negative bacteria gram staining principle this second microbiology lecture will explain the properties of gram positive and gram negative bacteria.
June 12, 2018 by sagar aryal gram staining is the common, important, and most used differential staining techniques in microbiology, which was introduced by danish bacteriologist hans christian gram in 1884. Chart and diagram slides for powerpoint beautifully designed chart and diagram s for powerpoint with visually stunning graphics and animation effects. About science prof online powerpoint resources science prof online spo is a free science education website that provides fullydeveloped virtual science classrooms, sciencerelated powerpoints, articles and images. Bacterial characteristics gram staining video khan academy. Gram staining of bacteria from environmental sources. Bacterial classification, structure and function introduction the purpose of this lecture is to introduce you to terminology used in microbiology. The adobe flash plugin is needed to view this content. Ppt gram staining powerpoint presentation free to download id. Ppt gram stain powerpoint presentation, free download. View and download powerpoint presentations on gram staining ppt. Feb 03, 2015 gram staining gram staining is most widely used differential staining in microbiology. Gram staining lab 3 hans christian gram developed gram staining in 1844 the reagents primary stain. Mackie objectives be able to discuss your results of the gram staining lab.
Gram stain definition of gram stain by the free dictionary. Gram staining a important technique a staining technique used to classify bacteria. Gram staining tips page 2 of 2 some specimens on preparation will have areas on the slide that are thicker than others. Gram negative bacteria do not retain the dark blue color. Rapid presumptive diagnosis of diseases such as bacterial meningitis. Gram stain, a widely used microbiological staining technique that greatly aids in the identification and characterization of bacteria. Gram staining is the property of its rightful owner. It includes introduction, history,principle, method of gram staining procedure slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Gram staining is a simple procedure if done with precision and accuracy can guide in diagnosis of several bacterial infections. Staphylococcus epidermidis a grampositive cocci found on the. Gram staining is based on the ability of the bacterial cell wall to retain the crystal violet dye.
Acidic dyes have a negative charge and are repelled by the negatively charged cell walls. Developed gram staining technique which is unique to bacterial cells due to their cell wall construction. The gram stain is a classical and important staining technique that remains widely used by environmental microbiologists. Apr 04, 2016 a staining technique used to classify bacteria. Define gram staining as the predominant method of bacterial investigation. Decolourization with alcohol or acetone, and counter staining with safranin. Worlds best powerpoint templates crystalgraphics offers more powerpoint templates than anyone else in the world, with over 4 million to choose from. Free class materials on the bacterial cell wall and differential staining, including lecture ppt, class notes. Gram staining method, the most important procedure in microbiology, was developed by danish physician hans christian gram in 1884. Gram staining or grams method is a method of differentiating bacterial species into two large groups grampositive and gramnegative. Gramstain mohammad rahbar reporting 4 issue a written preliminary report with the gram s stain results 5 telephonically notify the ordering provider or ward of. Staining, therefore, is of primary importance for the. Differentiate between simple and differential stains.
To learn the techniques of gram staining, nigrosin staining and koh test. A free powerpoint ppt presentation displayed as a flash slide show on id. Gram stain or gram staining, also called gram s method, is a method of staining used to distinguish and classify bacterial species into two large groups gram positive and gram negative. How to prepare a bacterial smear for gram staining. Jan 08, 2020 the gram staining technique is the most important and widely used microbiological differential staining technique. First lab in the series on identifying unknown bacteria. How it works gram positive bacteria have a thick layer ofpeptidoglycanup to 90%in their cell wall gram negative bacteria have a thinner layer 5 20%of this glycoprotein. This differential staining procedure separates most bacteria into two groups on the basis of cell wall composition. Gramnegative bacteria have a thin peptide layer and space surrounding it so the cell wall is.
Jul 01, 2019 microbiology lecture 2 gram positive and gram negative bacteria gram staining principle this second microbiology lecture will explain the properties of gram positive and gram negative bacteria. The gram stain is the most important staining procedure in microbiology. Ppt gramstain powerpoint presentation free to download. How can you tell a bacterial cell from normal body cells when a possible infection has taken place slideshow 3147360 by dyllis. Winner of the standing ovation award for best powerpoint templates from presentations magazine. Gram stain synonyms, gram stain pronunciation, gram stain translation, english dictionary definition of gram stain.
Skin infection grampositive cocci intraabdominal infections intraabdominal infections gramnegative bacilli gram. Hans christian joachim gram the gram stain was devised by the danish physician, hans christian joachim gram, while working in berlin in 1883. Demonstration of ziehl neelsen staining, a method of acid fast staining. Bacterial staining gram staining is the property of its rightful owner. Gram staining 2 free download as powerpoint presentation. The addition of grams iodine, which binds to crystal violet and traps it in the cell. The name comes from its inventor, hans christian gram. Students learn how to create a bacterial smear and preform the gram stain, with. Histologic staining options available include, but are not limited to. Four basic steps of gram staining applying a primary stain crystal violet to a heatfixed smear of a bacterial culture.
Biology ppts videos microbiology mcqs microbiology notes. A rapid method for distinction between gramnegative and grampositive bacteria by means of a 3% solution of potassium hydroxide is tested on 71. This can result in areas that are under decolorized, thus organisms that are truly gram negative will appear gram positive. Basic microbiology lab reporting gram staining free download as powerpoint presentation. Objectives to learn the technique of smear preparation. Selection of empirical antibiotics based on gram stain finding. Gram staining is an empirical method of differentiating bacterial species into two large groups gram positive and gram negative based on the chemical and physical properties of their cell walls. Gramstaining is a differential staining technique that uses a primary stain and a secondary counterstain to distinguish between grampositive and gramnegative bacteria. Ppt gram stain powerpoint presentation free to view id. Ppt the gram stain powerpoint presentation free to. If so, share your ppt presentation slides online with. Our new crystalgraphics chart and diagram slides for powerpoint is a collection of over impressively designed datadriven chart and editable diagram s guaranteed to impress any audience. The gram stain is a direct method, since the cells themselves retain dye. Find highquality gram stain stock photos and editorial news pictures from getty images.
Bacterial characteristics gram staining video khan. This video demonstrates the gram staining method for identifying unknown bacter. Microbiology lecture 2 gram positive and gram negative. Screening of the quality of the clinical specimens such as sputum that should contain many. Gram staining free download as powerpoint presentation. Gram staining differentiates the bacteria into 2 groups. Theyll give your presentations a professional, memorable appearance the kind of sophisticated look that.
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